Contact with potentially pathogenic microbes found on different surfaces from everyday items to health care settings (e.g. door handles, medical equipment, near-patient surfaces) may potentially harm our well-being. A promising method to reduce the spreading of unwanted microbes on surfaces is the development of antimicrobial surfaces containing photocatalysts that induce the degradation of organic matter under specific illumination conditions. One suitable class of photocatalysts is metal oxides such as TiO2 or ZnO. Two possibilities exist to increase the photocatalytic activity of metal oxide materials: (i) increasing the specific surface area of the material and (ii) implementing additives such as noble metals to promote charge separation during photoexcitation. In this research ZnO nanoparticles were chosen as the photocatalyst and Ag as the noble metal to prepare photocatalytically active antibacterial surfaces. For evaluation of time and illumination dependent antibacterial properties of prepared surfaces, viability test towards gram-negative model organism Escherichia coli was implemented.