Environmental and Experimental Factors Affecting Efficacy Testing of Nonporous Plastic Antimicrobial Surfaces

Test methods for efficacy assessment of antimicrobial coatings are not modelled on a hospital environment, and instead use high humidity (>90%) high temperature (37 °C), and no airflow. Therefore, an inoculum will not dry, resulting in an antimicrobial surface exhibiting prolonged antimicrobial activity, as moisture is critical to activity. Liquids will dry quicker in a hospital ward, resulting in a reduced antimicrobial efficacy compared to

The AMiCI WG2 workshop “Antimicrobial Coatings Applied in Healthcare Settings – Efficacy Testing”, Berlin, Germany

On 7-8 June 2018, the AMiCI WG2 workshop was held, combined with the Action Core Group Meeting, at the Bundesanstalt für Materialforschung und -prüfung (BAM) in Berlin, Germany. The European Union COST Action AMiCI  brings together experts on the synthesis, use and assessment of unwanted side effects of antimicrobial coatings. The project is coordinated by dr. Francy Crijns (Zuyd University of Applied Sciences and Technology,

WG2 Workshop in Berlin – Antimicrobial Coatings Applied in Healthcare Settings – Efficacy Testing

The WG2 Workshop on “Antimicrobial Coatings Applied in Healthcare Settings – Efficacy Testing” will be held in Federal Institute for Materials Research and Testing (BAM), Berlin, Thursday 7th of June 2018   The program of the Workshop 8:30 Registration 9:00 Welcome by representative of Federal Institute for Materials Research and Testing (BAM) 9:15 Introduction to AMiCI Francy Crijns, Zuyd University of Applied Sciences, The Netherlands 9:30 Objectives of WG2:

Comparative performance of a panel of commercially available antimicrobial nanocoatings in Europe

Background Bacterial resistance against the classic antibiotics is posing an increasing challenge for the prevention and treatment of infections in health care environments. The introduction of antimicrobial nanocoatings with active ingredients provides alternative measures for active killing of microorganisms, through a preventive hygiene approach. Purpose The purpose of this study was to investigate the antimicrobial activity of a panel of antimicrobial coatings available on the

Mechanism of copper surface toxicity in Escherichia coli O157:H7 and Salmonella involves immediate membrane depolarisation followed by slower rate of DNA destruction which differs from that observed for Gram-positive bacteria

We have reported previously that copper I and II ionic species, and superoxide but not Fenton reaction generated hydroxyl radicals, are important in the killing mechanism of pathogenic enterococci on copper surfaces. In this new work we determined if the mechanism was the same in non-pathogenic ancestral (K12) and laboratory (DH5α) strains, and a pathogenic strain (O157), of Escherichia coli. The pathogenic strain exhibited prolonged

Minimum information about a biofilm experiment (MIABiE): standards for reporting experiments and data on sessile microbial communities living at interfaces

The minimum information about a biofilm experiment (MIABiE) initiative has arisen from the need to find an adequate and scientifically sound way to control the quality of the documentation accompanying the public deposition of biofilm-related data, particularly those obtained using high-throughput devices and techniques. Thereby, the MIABiE consortium has initiated the identification and organization of a set of modules containing the minimum information that needs

A new way to find dielectric properties of liquid sample using the quartz crystal resonator (QCR)

The main objective of this article is to demonstrate by performing experimental measurements how the equivalent capacitance C0 changes when a fluid sample such as water is in contact with the crystal and to relate this change with the relative permittivity of the fluid. These measurements were compared with simulations of traditional models like Butterworth–Van Dyke (BVD). To obtain the change of C0 when the

Screening and characterization of surface-tethered cationic peptides for antimicrobial activity.

There is an urgent need to coat the surfaces of medical devices, including implants, with antimicrobial agents to reduce the risk of infection. A peptide array technology was modified to permit the screening of short peptides for antimicrobial activity while tethered to a surface. Cellulose-amino-hydroxypropyl ether (CAPE) linker chemistry was used to synthesize, on a cellulose support, peptides that remained covalently bound during biological assays.

Efficacy Assessment of Treated Articles: A guidance

This report provides guidance how to test efficacy of articles or material treated with biocides. There are two major groups: The first where the treatment is intended to protect the article itself. To prove efficacy, the material/article has to be tested against an untreated variant. In the second group, the treatment is intended to introduce new properties, e.g. to provide hygienic or insect repellency-functions. The

Phenotypic and molecular characterisation of multidrug-resistant Salmonella enterica serovar Hadar in Greece, from 2007 to 2010

All 120 strains of Salmonella enterica serovar Hadar isolated during 2007–2010 in Greece were characterized by phenotypic and molecular methods. High rates of resistance to nalidixic acid (92%) and low levels of ciprofloxacin resistance (88%) were observed. Pulsenet-pulsed field gel electrophoresis profile SHADXB.0001 was predominant in Greece (58%) as in Europe but PT1, a rare phage type in Europe, was frequent in Greece (56%). The